FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l01n01_6019.34100000035bf4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l01n01_6019.34100000035bf4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15403891
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG1352200.061.673021
TTTGTGT1467550.056.9726522
ACTTTGT442400.054.6328051
TTGTGTT1676150.051.265793
TAACTTT238000.046.9398421
TGTGTTT1844800.046.775094
AACTTTG248000.045.4274942
GTGTTTG2193150.039.3875665
TTGAGTC180150.031.8730494
TTTGAGG639900.031.1799168
TTTGAGT606950.030.669563
GTTTGAG1619500.029.8911232
TGTTTGA2929900.029.839951
GTTTGAA180950.029.2371442
GTTTGAC573100.027.8232127
TTTGACC173550.026.9152348
TTGAGGG400050.026.2362739
TTGACCC106800.024.7577769
TTGAGTG356650.023.7692834
TTTGAGA243050.023.2618268
Produced by FastQC (version 0.11.2)