FastQCFastQC Report
Thu 15 Oct 2015
C8364ACXX_l01n01_3522.34100000035b7d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8364ACXX_l01n01_3522.34100000035b7d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14950464
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGTGTTTGAGGGAGGTAGAATTCCACGTGTAGCAGTGAAATGCGTAG160010.10702677856687258No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGTG1426150.067.894181
TTTGTGT1535900.063.1383932
ACTTTGT386350.059.434171
TAACTTT154750.059.1871341
AACTTTG159000.057.931922
TTGTGTT1786900.055.8252143
TGTGTTT1871400.053.573984
GTGTTTG2167000.046.3122525
TTGAGTC234350.037.0265124
TTTGAGG621700.033.2107668
TTGAGGG326650.032.5418179
GTTTGAG1704750.032.505367
TGTTTGA3093250.032.4183126
TTTGAGT698000.032.400873
TTGAGGC101450.030.5584649
TGAGTCC173850.029.71155
TCCGATC13550.029.4313627
GTTTGAC589100.028.964067
TTTGATG279400.028.5975028
TGAGTGT102000.028.5318985
Produced by FastQC (version 0.11.2)