FastQCFastQC Report
Tue 29 Sep 2015
C7W8DACXX l08n01 n_90_3.34100000035028.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7W8DACXX l08n01 n_90_3.34100000035028.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14287060
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC1476031.0331236797493677TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT172800.042.19624742
TCGTATG174200.041.98203343
ACGTCTG177650.041.7004515
CACGTCT178450.041.4504714
GTATGCC176100.041.4412245
CACACGT183350.040.48995212
ATCGGAA184600.040.3132672
AGTCACC180800.040.2990228
ACACGTC184200.040.24204313
CGTCTGA185300.039.9181716
TGTAATC182050.039.50055736
TAATCTC182000.039.42487338
TCTCGTA185150.039.4070541
CAGTCAC186050.039.34161827
GTCTGAA192600.038.45189717
GTCACCT192050.038.2781429
CCAGTCA190950.038.24958426
TTGTAAT190150.038.05484435
CTTGTAA189600.037.99989334
TCCAGTC193950.037.87889525
Produced by FastQC (version 0.11.2)