FastQCFastQC Report
Tue 29 Sep 2015
C7W8DACXX l08n01 n_90_1.34100000034fe6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7W8DACXX l08n01 n_90_1.34100000034fe6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14860809
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC1185310.7976079902514055TruSeq Adapter, Index 10 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT140150.041.3184742
TCGTATG140500.041.2255343
ACGTCTG143450.041.08799415
CACGTCT146150.040.31353414
GTATGCC144750.039.92290545
GCTTATC146100.039.39388336
ATCGGAA151450.039.3335272
AGTCACT147050.039.3116628
TCACTAG147350.039.21582830
TATCTCG147250.039.13219539
CACACGT151250.038.90957612
ACACGTC151600.038.83458713
CGTCTGA152550.038.3862916
TAGCTTA149000.038.35715534
CACTAGC150700.038.32785431
TTATCTC150600.038.30654538
CTTATCT151550.038.02175537
CAGTCAC152800.037.88945427
CCAGTCA154500.037.44341726
TCTCGTA156450.037.0001341
Produced by FastQC (version 0.11.2)