FastQCFastQC Report
Tue 29 Sep 2015
C7W8DACXX l08n01 n_5_3.34100000034f02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7W8DACXX l08n01 n_5_3.34100000034f02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20711305
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC943510.45555313873268727TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG117650.039.7450828
TCGTATG125050.037.3567643
ACGTCTG129400.037.2732815
CTCGTAT125700.037.1907742
TATCTCG127300.036.817239
CGTCTGA132000.036.47093616
CACGTCT133250.036.2638714
CACACGT133200.036.19303512
CGTATGC131600.035.58293544
GTATGCC132500.035.3595945
ACACGTC139050.034.84831613
ATCGGAA138600.034.653072
CGCCAAT133000.034.20784433
TCTCGTA138000.034.07230441
GTCACGC138350.034.0261129
TCGGAAG143150.033.6302033
GCCAATA137400.032.9990434
CAGTCAC142900.032.92592227
TCACGCC143650.032.67616730
GATCGGA149150.032.4335671
Produced by FastQC (version 0.11.2)