FastQCFastQC Report
Tue 29 Sep 2015
C7W8DACXX l08n01 n_5_2.34100000034ecd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7W8DACXX l08n01 n_5_2.34100000034ecd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24234142
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC2167610.8944447053252391TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG269600.040.60378315
CTCGTAT266300.040.48364642
TCGTATG266450.040.43492543
CACGTCT273950.040.1068614
ATCGGAA278700.039.5766642
ACACGTC279300.039.27417413
CGTCTGA277150.039.2541616
CACACGT279200.039.2480312
GTATGCC274850.039.19241745
GTCACAC279950.038.40497629
GATCGGA294000.037.77941
TCTCGTA285750.037.7600341
TCGGAAG293000.037.6604653
CACAGTG287900.037.42046733
ACACAGT285750.037.40279832
CAGTCAC289300.037.1862427
AGTCACA289800.036.8744628
CCAGTCA294350.036.555926
CGTATGC295100.036.48639744
TCCAGTC303850.035.71695725
Produced by FastQC (version 0.11.2)