FastQCFastQC Report
Tue 29 Sep 2015
C7W8DACXX l08n01 n_5_1.34100000034e97.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7W8DACXX l08n01 n_5_1.34100000034e97.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19207003
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC2248191.1705053620286308TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG274500.042.37893715
CTCGTAT267800.042.12240242
CACGTCT277600.041.9137914
TCGTATG270250.041.8228643
GTATGCC270600.041.77061545
ACACGTC279550.041.57313513
AGTCACT274750.041.50662628
ACTGACC273900.041.37838732
CACACGT282200.041.2146312
ATCGGAA284700.041.1687972
CAGTCAC283550.040.26429727
CACTGAC283250.040.24360331
CGTCTGA290400.039.9656416
CCAGTCA288150.039.55904826
GTCTGAA294200.039.5106117
GTCACTG292550.039.2965429
AGCACAC296350.039.2467310
TCCAGTC292600.039.08896325
TCACTGA295500.038.6755930
ACTCCAG296550.038.66056423
Produced by FastQC (version 0.11.2)