FastQCFastQC Report
Tue 29 Sep 2015
C7W8DACXX l08n01 k_90_3.34100000034fbf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7W8DACXX l08n01 k_90_3.34100000034fbf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16385650
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC10939716.676396725183316TruSeq Adapter, Index 9 (100% over 51bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186380.11374586909887614No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG1225050.044.13326628
ACGTCTG1243750.044.13042415
CGTCTGA1243200.044.084816
ATCGGAA1247400.044.0535932
CTCGTAT1227700.044.02091242
GTATGCC1228850.043.97209545
CACGTCT1247950.043.96928414
GTCACGA1225300.043.9387829
TCGTATG1232700.043.92286743
CACACGT1247900.043.9205712
GATCGGA1258200.043.87141
TCGGAAG1253700.043.851963
ACACGTC1249700.043.84290713
CGTATGC1237300.043.69228744
TCACGAT1233300.043.6572830
TCTCGTA1239050.043.61847341
CCAGTCA1242800.043.47432726
CAGTCAC1244250.043.4616427
GCACACG1264100.043.37194411
CACGATC1236250.043.36963331
Produced by FastQC (version 0.11.2)