FastQCFastQC Report
Tue 29 Sep 2015
C7W8DACXX l08n01 k_90_2.34100000034f7c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7W8DACXX l08n01 k_90_2.34100000034f7c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17533087
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC3593582.049599137904238TruSeq Adapter, Index 8 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG412550.042.94804415
CTCGTAT407500.042.8746142
TCGTATG408250.042.87077743
CACGTCT415900.042.58588414
CGTCTGA416350.042.5290516
ATCGGAA418750.042.28532
GTATGCC414350.042.23070545
CACACGT419950.042.13232412
ACACGTC421200.042.01263413
GTCACAC416600.041.8174229
TCTCGTA419200.041.67939441
TCGGAAG426150.041.5563053
CAGTCAC418450.041.47481527
GATCGGA430950.041.368551
AATCTCG417650.041.14234539
TCACACT424200.041.0995730
CACACTT425400.041.0090331
AGTCACA421050.040.9960128
GTCTGAA433750.040.76074617
CCAGTCA425750.040.7319726
Produced by FastQC (version 0.11.2)