FastQCFastQC Report
Tue 29 Sep 2015
C7W8DACXX l08n01 k_90_1.34100000034f39.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7W8DACXX l08n01 k_90_1.34100000034f39.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14266121
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC4850803.4002235085486796TruSeq Adapter, Index 7 (100% over 51bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163310.114474004531435No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT543750.043.65007442
TCGTATG545850.043.5601543
ACGTCTG557250.043.4758415
CACGTCT558200.043.40184814
GTATGCC548100.043.3626345
CGTCTGA560000.043.19806716
ATCGGAA561450.043.1225662
CACACGT562550.043.0182512
ACACGTC562400.043.00572213
AGTCACC551750.042.8546428
GATCGGA569000.042.755441
TCTCGTA556500.042.6869941
CAGTCAC557400.042.62867427
TCGGAAG572450.042.3017963
CCAGTCA562450.042.22192826
GTCTGAA571600.042.16397517
TCCAGTC570350.041.9614925
TCTGAAC576500.041.78775418
ACTCCAG571550.041.78692223
GCACACG580300.041.74894311
Produced by FastQC (version 0.11.2)