FastQCFastQC Report
Tue 29 Sep 2015
C7W8DACXX l08n01 k_5_3.34100000034e47.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7W8DACXX l08n01 k_5_3.34100000034e47.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17987126
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC891710.49574901515672937TruSeq Adapter, Index 3 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTAGG107100.038.95138532
CACTTAG107200.038.74820731
CTCGTAT110200.038.31920642
TCGTATG111000.038.11252243
CTTAGGC111300.037.2995733
ACGTCTG115100.037.2912315
TTAGGCA111950.036.8424334
CACGTCT118350.036.36221714
CACACGT119000.036.18250312
CGTCTGA118800.036.07299416
TCACTTA116050.035.8721230
ATCGGAA120350.035.6644822
AGTCACT117500.035.640528
GTATGCC119050.035.51798645
TAGGCAT118750.034.78772435
GTCACTT121900.034.7786329
CGTATGC121850.034.7557744
ACACGTC124550.034.66050313
TCTCGTA124800.033.9813241
CAGTCAC126150.033.3733727
Produced by FastQC (version 0.11.2)