FastQCFastQC Report
Tue 29 Sep 2015
C7W8DACXX l08n01 k_5_2.34100000034df5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7W8DACXX l08n01 k_5_2.34100000034df5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19650328
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC721450.36714399881772963TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG95900.036.95563543
CTCGTAT96550.036.81699842
ACGTCTG102500.035.57765215
TATCTCG100350.035.26903539
CACGTCT103650.035.2046214
CGTCTGA106200.034.23221216
CACACGT107900.033.67202412
GTATGCC106000.033.41443645
ATCGGAA108950.033.409462
AGTCACC107450.033.0456128
ACACGTC110950.032.7058313
GTATCTC109050.032.62033538
CAGTCAC112550.031.5273327
TCTCGTA113800.031.256341
CGATGTA113900.030.99537734
ACCGATG115150.030.77568232
CACCGAT115150.030.54174431
CCGATGT116900.030.35346433
GATCGGA122250.030.2259481
GTCTGAA121700.029.98322917
Produced by FastQC (version 0.11.2)