FastQCFastQC Report
Tue 29 Sep 2015
C7W8DACXX l08n01 k_5_1.34100000034dce.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7W8DACXX l08n01 k_5_1.34100000034dce.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21551519
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG32450.017.26682543
CTCGTAT33300.016.75457842
ACGTCTG37300.016.34445215
CGTCTGA39950.015.09134416
CACACGT42300.014.78476212
CACGTCT40700.014.592154514
GTATGCC41400.014.29540145
ATCGGAA43700.013.59040452
CGTATGC41750.013.58225544
ACACGTC47900.013.10323713
CAGTCAC49750.012.21187527
AGTCACA49750.011.89560228
GTCACAT49150.011.81190629
TCACGAT48800.011.75722535
TCTCGTA48500.011.7357541
TCGGAAG53750.011.3004363
GATCGGA57200.010.9122851
ATCACGA55250.010.30366934
GGGGGAT57750.010.2230671
GTCTGAA62150.09.95408817
Produced by FastQC (version 0.11.2)