FastQCFastQC Report
Tue 29 Sep 2015
C7W8DACXX l07n01 t0_2.34100000034e3a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7W8DACXX l07n01 t0_2.34100000034e3a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14017930
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC661140.4716388225651006TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG84600.036.8894143
CTCGTAT85200.036.6259642
ACGTCTG87850.036.519815
TATCTCG85600.036.29776439
CGTATGC87600.035.65175644
CACGTCT90050.035.5526414
CGTCTGA90450.035.51978316
ATCGGAA91500.035.4308172
CACACGT91800.035.11909512
GTATGCC90800.034.5200745
ACACGTC95300.033.49868413
TCGGAAG98050.033.1098333
TCTCGTA94650.033.04083641
AGTCACC94800.032.9212328
GTATCTC95200.032.66138
GATCGGA101150.032.4439541
CGATGTA96550.032.22854234
CAGTCAC97650.032.0975127
CACCGAT98250.031.87813831
CCGATGT98300.031.83789833
Produced by FastQC (version 0.11.2)