FastQCFastQC Report
Tue 29 Sep 2015
C7W8DACXX l07n01 t0_1.34100000034e10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7W8DACXX l07n01 t0_1.34100000034e10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15356621
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG20500.011.74305215
CTCGTAT19350.011.27810542
TCGTATG21000.011.0362843
CGTATGC21950.010.96867144
CGTCTGA22750.09.88939316
CACGTCT24400.09.58946814
GTATGCC26800.08.90014445
CACACGT28900.08.7187512
ACACGTC29100.08.658813
ATCGGAA26900.08.4469852
CCCGTAT8650.08.0776491
TCACGAT28400.08.00115435
CGGGGAT35850.07.9217441
GGGAAAT40300.07.6062971
TCTCGTA29650.07.43617541
TCGGAAG34000.07.08006953
GGGAGAT64700.07.07181931
GTCGGAT20400.07.0711361
GTCACAT33150.06.991376429
ATCACGA35200.06.967187434
Produced by FastQC (version 0.11.2)