FastQCFastQC Report
Tue 29 Sep 2015
C7W8DACXX l07n01 n_10_3.34100000034fcc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7W8DACXX l07n01 n_10_3.34100000034fcc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16632446
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC3094871.860742551035488TruSeq Adapter, Index 9 (100% over 51bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT852140.5123359486632333No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA424170.2550256288221227No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG349000.043.26834528
TCGTATG353300.042.85000243
CTCGTAT353400.042.81870342
ACGTCTG359000.042.66530215
CGTATGC357850.042.31145544
CGTCTGA361950.042.27405516
CACGTCT362750.042.19323314
GTCACGA355800.042.17579729
ATCGGAA364250.041.9743462
GTATGCC361000.041.91997545
CACACGT365450.041.85498412
TCTCGTA364300.041.52557441
TCACGAT361700.041.4751430
ACACGTC369500.041.44493513
TCGGAAG371050.041.205113
CAGTCAC368650.040.9611727
GTCTGAA373900.040.8808417
GATCGGA375700.040.8788531
ACGATCA369350.040.28349332
GCACACG380450.040.26390511
Produced by FastQC (version 0.11.2)