FastQCFastQC Report
Tue 29 Sep 2015
C7W8DACXX l07n01 n_10_2.34100000034f89.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7W8DACXX l07n01 n_10_2.34100000034f89.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13571614
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC2057521.5160466544362372TruSeq Adapter, Index 8 (100% over 51bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT385080.28373928112013796No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA171940.1266909005811689No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT233100.042.4388642
TCGTATG234550.042.2190743
ACGTCTG240050.041.96056415
CGTCTGA239900.041.9305316
CGTATGC237300.041.81514444
CACACGT241850.041.57277312
CACGTCT242500.041.5644714
GTATGCC241150.041.111645
GTCACAC240850.041.04120629
ATCGGAA245400.040.9710732
AATCTCG238900.040.9194339
ACACGTC246850.040.7395213
TCGGAAG248750.040.4464383
AGTCACA245150.040.15611628
CAGTCAC247100.040.0843527
TCACACT247350.039.97179830
CACACTT247850.039.89832731
TCTCGTA248650.039.85751341
GTCTGAA252650.039.76107817
GATCGGA255350.039.670751
Produced by FastQC (version 0.11.2)