FastQCFastQC Report
Tue 29 Sep 2015
C7W8DACXX l07n01 n_10_1.34100000034f46.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7W8DACXX l07n01 n_10_1.34100000034f46.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12778939
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC1663181.3015008523008054TruSeq Adapter, Index 7 (100% over 51bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT546800.42789154874281815No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA204340.15990372909675835No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG191750.041.9268243
CTCGTAT191750.041.8868642
ACGTCTG197100.041.4804315
CGTATGC194250.041.37563744
CGTCTGA199500.040.9475916
CACGTCT199550.040.92605614
CACACGT199850.040.91994512
ACACGTC203250.040.20222513
GTATGCC200200.040.16983445
ATCGGAA204250.039.9943772
AGTCACC201550.039.77843528
TCTCGTA203000.039.6877741
TCGGAAG207950.039.304413
CAGTCAC205100.039.2095327
GATCGGA209850.039.009711
GTCTGAA209650.038.95442217
GCACACG215050.038.11136611
CCAGTCA211600.037.94127726
AGCACAC216200.037.85661710
TCTGAAC218150.037.40829518
Produced by FastQC (version 0.11.2)