FastQCFastQC Report
Tue 29 Sep 2015
C7W8DACXX l07n01 k_20_2.34100000035042.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7W8DACXX l07n01 k_20_2.34100000035042.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14086748
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149330.10600743336929147No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG17800.025.03017828
TCGTATG21300.021.12829843
CTCGTAT21800.020.74383242
ACGTCTG25350.018.63710215
CACGTCT26550.017.455814
CGTCTGA27050.017.21631216
CGTATGC28100.016.49586344
GTATGCC28300.015.90334745
CACACGT30400.015.54086112
ACGGCTA30400.014.72838432
ATCGGAA31750.014.6674432
ACACGTC33250.014.41171813
GTCACGG33000.013.97843929
TCTCGTA34600.013.33009841
CAGTCAC36000.013.25049427
CACGGCT35350.013.04825331
TCGGAAG35900.012.8465713
GTCTGAA40000.012.03624117
CCAGTCA39750.011.94384226
GCACACG43900.011.63297211
Produced by FastQC (version 0.11.2)