FastQCFastQC Report
Tue 29 Sep 2015
C7W8DACXX l07n01 k_10_3.34100000034ef4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7W8DACXX l07n01 k_10_3.34100000034ef4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12975001
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC1764651.3600384308255544TruSeq Adapter, Index 6 (100% over 51bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT411830.31740267303254927No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA166570.12837763943139582No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG203250.042.6792328
CTCGTAT206950.041.90891342
TATCTCG208300.041.74579239
ACGTCTG212400.041.74434715
TCGTATG208750.041.63841643
CGTCTGA214450.041.31382816
CACGTCT215050.041.29271714
ATCGGAA216950.041.0234682
CACACGT217300.040.81244712
GTATGCC214050.040.58812345
CGCCAAT210850.040.5498533
CGTATGC215350.040.3100544
ACACGTC220850.040.16660313
GCCAATA212400.040.07385334
TCGGAAG222600.039.9822163
GTCACGC219350.039.76206229
TCTCGTA218450.039.75432641
CAGTCAC219300.039.66683627
GATCGGA226950.039.364341
CCAATAT217250.039.17620535
Produced by FastQC (version 0.11.2)