FastQCFastQC Report
Tue 29 Sep 2015
C7W8DACXX l07n01 k_10_2.34100000034eb0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7W8DACXX l07n01 k_10_2.34100000034eb0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13247199
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC2468341.8632920061063474TruSeq Adapter, Index 5 (100% over 51bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT282300.2131016526587998No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT278950.042.4716242
ACGTCTG282350.042.47062715
CGTCTGA282400.042.3356416
TCGTATG280500.042.30556543
CACGTCT284650.042.21440514
CGTATGC282850.041.9461244
CACACGT286100.041.90466312
ATCGGAA287650.041.811822
GTATGCC285850.041.53045345
ACACGTC289350.041.50396313
TCGGAAG290050.041.450343
GTCACAC286150.041.4252629
ACACAGT287700.040.93980432
CAGTCAC290100.040.78302827
AGTCACA289600.040.7375128
TCTCGTA291300.040.7026541
GTCTGAA293550.040.64328817
GATCGGA297500.040.585861
CACAGTG291050.040.47631533
GCACACG299900.040.07392511
Produced by FastQC (version 0.11.2)