FastQCFastQC Report
Tue 29 Sep 2015
C7W8DACXX l07n01 k_10_1.34100000034e7d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7W8DACXX l07n01 k_10_1.34100000034e7d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14203967
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC1304680.9185321255674559TruSeq Adapter, Index 4 (100% over 51bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT638830.44975463544797023No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA279450.19674081191543177No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT156000.040.6705542
ACGTCTG160350.040.61841215
TCGTATG156600.040.6049343
CGTCTGA161400.040.29841216
CACGTCT162150.040.15363714
ATCGGAA163500.039.8490522
CACACGT163850.039.68168612
CGTATGC160100.039.66103744
AGTCACT161150.039.61303328
GTATGCC162200.039.23188445
ACTGACC162850.039.14148732
ACACGTC166600.039.1210713
TCGGAAG169650.038.4442633
AATCTCG163800.038.37714839
TCTCGTA166700.038.14100341
CAGTCAC168700.037.8918127
GATCGGA174650.037.489041
GTCTGAA174150.037.38680617
CACTGAC172650.037.08979831
GACCAAT170750.036.7356435
Produced by FastQC (version 0.11.2)