FastQCFastQC Report
Tue 29 Sep 2015
C7W8DACXX l06n01 n_30_3.3410000003511a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7W8DACXX l06n01 n_30_3.3410000003511a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15195911
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC2692061.7715686805483397TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG301400.042.51225343
CGTCTGA306050.042.48260516
CTCGTAT301500.042.48169342
ACGTCTG306700.042.46592715
CACGTCT310150.041.96452714
CGTATGC305300.041.90286644
GTATGCC305700.041.87135345
ATCGGAA312600.041.78652
CACACGT312350.041.5894512
ACACGTC314000.041.41390213
AATCTCG307650.041.28876539
TCGGAAG316600.041.237243
AGTCACC311000.041.1596928
TCTCGTA312550.041.01577441
TGTAATC309650.040.97122636
CAGTCAC313050.040.9045327
GATCGGA321250.040.8617741
GTAATCT310400.040.85048737
TAATCTC313050.040.5262438
GTCTGAA323850.040.0989717
Produced by FastQC (version 0.11.2)