FastQCFastQC Report
Tue 29 Sep 2015
C7W8DACXX l06n01 n_30_2.341000000350e2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7W8DACXX l06n01 n_30_2.341000000350e2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15041465
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC1209430.8040639658437525TruSeq Adapter, Index 11 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG148200.041.220428
ACGTCTG156650.040.21445515
CGTCTGA156450.040.17957316
CTCGTAT152750.039.90046742
CACGTCT158450.039.85700614
TCGTATG153650.039.8295443
CGTATGC153200.039.74092544
ACGGCTA156150.039.0612732
CACACGT161650.039.05369612
ATCGGAA162400.038.8733372
GTATGCC159450.038.32544345
ACACGTC164700.038.24851613
GTCACGG162500.037.8837829
TCTCGTA162650.037.5548441
TCGGAAG168400.037.541743
GATCGGA171400.037.014021
CAGTCAC165850.037.01003327
CACGGCT166500.036.85007531
GTCTGAA174600.035.89974617
GGCTACA167750.035.71638534
Produced by FastQC (version 0.11.2)