FastQCFastQC Report
Tue 29 Sep 2015
C7W8DACXX l06n01 n_30_1.341000000350c8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7W8DACXX l06n01 n_30_1.341000000350c8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15601975
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC670260.42959945776095654TruSeq Adapter, Index 10 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT86900.038.0060842
TCGTATG88350.037.38388443
ACGTCTG91850.036.7423815
CGTATGC90200.036.6919744
CGTCTGA92500.036.386916
TATCTCG91400.036.159539
CACGTCT94250.035.83063514
CACACGT94300.035.64394412
GTCACTA94100.035.1736329
AGTCACT95750.034.63800428
TCTCGTA96200.034.5657741
TAGCTTA95100.034.49276734
ACACGTC98150.034.36039413
ATCGGAA98950.034.1735422
GTATGCC97700.033.968645
TCACTAG98250.033.87113630
GCTTATC99350.033.15278236
TCGGAAG103850.032.6911053
CACTAGC100900.032.6890431
TTATCTC101750.032.68036338
Produced by FastQC (version 0.11.2)