FastQCFastQC Report
Tue 29 Sep 2015
C7W8DACXX l06n01 n_15_3.3410000003501b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7W8DACXX l06n01 n_15_3.3410000003501b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19698107
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC801130.40670405536938137TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG102650.039.36672628
ACGTCTG110400.037.39567615
TCGTATG108550.037.12179643
CGTCTGA111850.036.97123316
CTCGTAT109150.036.95802742
TATCTCG109450.036.77449839
CACGTCT115100.035.927314
CGTATGC113550.035.4673844
CACACGT118550.035.03340512
ATCGGAA121200.034.4159132
CGCCAAT115950.033.9956433
ACACGTC123550.033.6338313
GTATGCC119700.033.60906245
GCCAATA117650.033.3514334
TCTCGTA123500.032.75479541
GTCACGC125600.032.38849329
TCGGAAG128650.032.335483
CAGTCAC126550.031.96756227
GATCGGA131750.031.7657641
TCACGCC132750.030.74564630
Produced by FastQC (version 0.11.2)