FastQCFastQC Report
Tue 29 Sep 2015
C7W8DACXX l06n01 n_15_2.34100000034fd9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7W8DACXX l06n01 n_15_2.34100000034fd9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18650801
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC1000780.536588214093325TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT122350.038.8361342
TCGTATG123250.038.6639343
CGTCTGA127550.037.8358316
ACGTCTG129350.037.5180415
CGTATGC128750.037.01226444
CACGTCT131700.036.88275514
ATCGGAA133700.036.414292
CACACGT133450.036.2970612
GTATGCC132700.036.01391245
GTCACAC135300.035.38884429
ACACGTC138650.035.03311513
TCTCGTA138250.034.48353641
ACACAGT137850.034.42085332
TCGGAAG143400.033.966813
CAGTCAC141100.033.774727
GATCGGA145850.033.6686861
CACAGTG141950.033.4108133
AGTCACA142200.033.32355528
GTCTGAA148900.032.38052717
CCAGTCA150500.031.79973826
Produced by FastQC (version 0.11.2)