FastQCFastQC Report
Tue 29 Sep 2015
C7W8DACXX l06n01 n_15_1.34100000034f96.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7W8DACXX l06n01 n_15_1.34100000034f96.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18732077
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC983760.5251740103353194TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG127650.039.1637443
CTCGTAT128500.038.74627342
CGTCTGA134400.038.08363316
ACGTCTG134600.037.97700515
CGTATGC133500.037.54869544
CACGTCT136200.037.5143514
ATCGGAA139850.036.8082472
CACACGT139550.036.64564512
AGTCACT136950.036.45696628
GTATGCC137800.036.37845645
ACTGACC137250.036.24361432
ACACGTC144650.035.3536113
AATCTCG139250.035.3188539
TCGGAAG148300.034.7868043
TCTCGTA144600.034.57222741
CAGTCAC147750.033.97483427
GATCGGA154450.033.5585141
CACTGAC150000.033.35825331
GTCTGAA154350.033.14668317
GACCAAT147100.033.08228335
Produced by FastQC (version 0.11.2)