FastQCFastQC Report
Tue 29 Sep 2015
C7W8DACXX l06n01 k_30_3.341000000350af.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7W8DACXX l06n01 k_30_3.341000000350af.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15139424
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC1411290.9321953067699272TruSeq Adapter, Index 9 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG163350.042.19139528
TCGTATG168150.040.85049443
CTCGTAT169050.040.59120642
ACGTCTG172150.040.5142415
CGTATGC169550.040.42029644
GTCACGA171050.040.0553329
CGTCTGA174650.039.93417416
CACGTCT175750.039.69716314
GTATGCC174500.039.35392445
CACACGT177800.039.2390712
ACACGTC179350.038.89995613
ATCGGAA179950.038.782632
TCTCGTA179400.038.3121141
TCACGAT179400.038.2410330
TCGGAAG184850.037.7789233
GATCGGA186000.037.6327971
CAGTCAC185350.037.29277427
ACGATCA185500.036.67687632
GTCTGAA192400.036.27340717
CCAGTCA192000.036.03627826
Produced by FastQC (version 0.11.2)