FastQCFastQC Report
Tue 29 Sep 2015
C7W8DACXX l06n01 k_30_2.34100000035085.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7W8DACXX l06n01 k_30_2.34100000035085.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14918869
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC14590809.780097941740758TruSeq Adapter, Index 8 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG1625500.044.16974615
CGTCTGA1624200.044.15246616
CACGTCT1627850.044.1239514
ATCGGAA1630400.044.1150632
GTATGCC1607050.044.0749245
TCGTATG1609050.044.0618643
CACACGT1628000.044.05986812
CTCGTAT1608750.044.02068742
GATCGGA1642400.044.0075451
ACACGTC1629650.044.0028413
CGTATGC1611650.043.94610644
TCGGAAG1636100.043.935253
AATCTCG1591850.043.8972639
TCTCGTA1616550.043.8305541
GTCACAC1613750.043.80974229
GCACACG1641700.043.70041311
CAGTCAC1615850.043.6734427
CACACTT1620100.043.65995431
TCACACT1620350.043.63670730
GTCTGAA1640950.043.6318517
Produced by FastQC (version 0.11.2)