FastQCFastQC Report
Tue 29 Sep 2015
C7W8DACXX l06n01 k_30_1.3410000003505f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7W8DACXX l06n01 k_30_1.3410000003505f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13656560
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC663800.4860667693767684TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT82650.038.70889342
TCGTATG83050.038.60542743
ACGTCTG87800.037.15614715
CGTCTGA88200.037.06416716
CACGTCT88400.036.9548614
CGTATGC87200.036.71652244
CACACGT90300.036.2766912
GTATGCC89600.035.78405445
ATCGGAA92150.035.4263272
ACACGTC93150.035.0218613
TCTCGTA92800.034.74179541
TCGGAAG94850.034.655083
AGTCACC93250.034.28674728
CAGTCAC97450.032.87829227
GATCGGA101150.032.597811
GTCTGAA103500.031.69380817
CCAGTCA102300.031.47350326
GCACACG104650.031.3882911
TCCAGTC110050.029.46172725
AGCACAC112050.029.43582210
Produced by FastQC (version 0.11.2)