FastQCFastQC Report
Tue 29 Sep 2015
C7W8DACXX l06n01 k_15_3.34100000034f6f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7W8DACXX l06n01 k_15_3.34100000034f6f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16917433
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC752380.4447365034636165TruSeq Adapter, Index 3 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTAGG94450.038.5659932
TCGTATG95650.038.3180543
CACTTAG95650.038.22407531
CTCGTAT97300.037.55142242
CGTATGC99050.037.07088544
CTTAGGC98750.037.0233633
ACGTCTG100700.036.86531415
TTAGGCA99900.036.25934634
CACGTCT102650.036.18691614
CGTCTGA104300.035.59287616
GTATGCC104400.035.30153345
TCACTTA104350.035.16761830
AGTCACT105850.034.62674328
CACACGT107650.034.52623412
TAGGCAT105050.034.5026735
ATCGGAA109450.034.06122
ACACGTC109600.033.99405713
GTCACTT109650.033.69348529
TCTCGTA109500.033.57307441
CAGTCAC112800.032.69273827
Produced by FastQC (version 0.11.2)