FastQCFastQC Report
Tue 29 Sep 2015
C7W8DACXX l06n01 k_15_2.34100000034f2c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7W8DACXX l06n01 k_15_2.34100000034f2c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18510975
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC999980.5402092542397146TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT125250.037.8834942
TCGTATG125850.037.70440743
ACGTCTG129800.037.3706915
CGTATGC127000.037.3629944
CACACGT131100.036.96529412
TATCTCG129300.036.59268639
CACGTCT133450.036.31484214
CGTCTGA134350.036.1218116
ATCGGAA136800.035.8197862
ACACGTC136050.035.5707513
GTATGCC135000.035.1166945
TCTCGTA138650.034.33578541
GATCGGA146350.033.7971271
TCGGAAG145150.033.6971933
CGATGTA139900.033.4826734
AGTCACC141750.033.4767628
GTATCTC144200.032.92082638
ATGTATC143250.032.74650636
GCACACG148750.032.7304111
CAGTCAC145200.032.69683527
Produced by FastQC (version 0.11.2)