FastQCFastQC Report
Tue 29 Sep 2015
C7W8DACXX l06n01 k_15_1.34100000034ee7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7W8DACXX l06n01 k_15_1.34100000034ee7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17467413
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG18300.012.78630843
CTCGTAT20450.011.99174642
ACGTCTG24000.010.87435415
CGTCTGA25600.010.10682216
CGTATGC23900.09.79035344
CACGTCT27200.09.76044814
CACACGT29550.09.59317412
GGGGTAT15200.08.8961021
ACACGTC33850.08.77333113
GTATGCC28550.08.74779245
ATCGGAA30750.08.4871552
TCACGAT31350.08.46825135
GGGGGAT44200.08.3620661
GTCGGAT23850.08.2209671
TCTCGTA32400.08.05493841
CGGGGAT48350.07.644331
GGGAAAT44650.07.47020151
CGGGCAT29400.07.43560741
TCGGAAG37100.07.3377133
GCGGGAT36850.06.84971671
Produced by FastQC (version 0.11.2)