FastQCFastQC Report
Tue 29 Sep 2015
C7W8DACXX l05n01 8nm-1.341000000350d5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7W8DACXX l05n01 8nm-1.341000000350d5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16920054
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC6838714.041777880850734TruSeq Adapter, Index 7 (100% over 51bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT379060.22403001787110136No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA173660.10263560624570112No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG759950.043.81466743
CTCGTAT761050.043.6943842
ACGTCTG781500.043.56643315
ATCGGAA783250.043.443122
CACGTCT787500.043.23151414
CGTCTGA787900.043.2011316
ACACGTC787800.043.1693613
GTCTGAA786400.043.1261917
TCGGAAG789100.043.1010933
CGTATGC773550.043.01248644
GTATGCC773700.042.9955645
TCTCGTA775400.042.90379341
CAGTCAC777800.042.8478127
AGTCACC773600.042.8013128
CGGAAGA799450.042.5318344
CACACGT799750.042.52150312
GCACACG802150.042.44756711
CCAGTCA785550.042.43940726
TCCAGTC794800.042.41548525
GATCGGA807800.042.3435971