FastQCFastQC Report
Tue 29 Sep 2015
C7W8DACXX l05n01 4nm-2.34100000035092.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7W8DACXX l05n01 4nm-2.34100000035092.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15990239
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC4327952.706619957337723TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG490250.043.162243
CTCGTAT491700.043.0143342
ACGTCTG503050.042.96497715
ATCGGAA505900.042.8207662
GTCACAC493200.042.60651829
CACGTCT509250.042.4463114
AGTCACA492400.042.43809528
CGTCTGA506750.042.39821616
GTCTGAA505750.042.35304317
ACACGTC509550.042.31976713
TCGGAAG512950.042.2103083
ACACAGT492950.042.0836632
TCTCGTA504250.041.95741741
GTATGCC505150.041.92615545
CGTATGC507150.041.75076744
CACAGTG499050.041.72255733
AGTGATC496600.041.71140336
CAGTCAC507200.041.61668427
CGGAAGA523000.041.4077954
ACAGTGA496850.041.40463334
Produced by FastQC (version 0.11.2)