FastQCFastQC Report
Tue 29 Sep 2015
C7W8DACXX l05n01 4nm-1.3410000003506b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7W8DACXX l05n01 4nm-1.3410000003506b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11398388
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC2950382.5884186430572464TruSeq Adapter, Index 4 (100% over 51bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136950.12014856837651079No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG351650.043.04498743
ATCGGAA362350.042.9769252
ACGTCTG362450.042.9712715
CTCGTAT352550.042.93997642
AATCTCG346150.042.57795339
CACGTCT365950.042.5602914
TCGGAAG366650.042.460633
AGTCACT357400.042.4500928
ACTGACC355000.042.4095132
CGTCTGA366250.042.39643516
ACACGTC368150.042.2448513
GACCAAT344100.042.21721335
GTCTGAA368550.042.01587317
CGTATGC361500.041.83532744
CAGTCAC364000.041.7420327
TCTCGTA363150.041.7184941
CACTGAC363850.041.64736631
GTATGCC363150.041.6365145
ACCAATC349450.041.53206336
CGGAAGA375000.041.509174