FastQCFastQC Report
Tue 29 Sep 2015
C7W8DACXX l05n01 32nm-3.34100000035177.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7W8DACXX l05n01 32nm-3.34100000035177.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14432429
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG970870.6727003472527043TruSeq Adapter, Index 15 (97% over 40bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT302660.20970828957481794No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG120900.040.6669745
CTCGTAT123300.040.07169744
AATCTCG125450.038.9166141
ACGTCTG134250.038.79480715
AGTCACA129250.038.459328
GTCACAT130700.038.23923529
ATCGGAA137700.037.6594472
GAATCTC131350.037.2884540
CACGTCT141100.036.9433214
TGTCAGA134050.036.94006735
TCGGAAG140800.036.8942073
CGTCTGA141500.036.88658516
ACACGTC143400.036.49198513
GTCTGAA143350.036.42624317
CAGTCAC138800.036.26709427
TCTCGTA138550.035.92076543
GTCAGAA135100.035.92024236
AGAATCT137650.035.38568539
ATGTCAG142500.034.9732834
CATGTCA143750.034.77873233
Produced by FastQC (version 0.11.2)