FastQCFastQC Report
Tue 29 Sep 2015
C7W8DACXX l05n01 32nm-2.3410000003516a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7W8DACXX l05n01 32nm-2.3410000003516a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14571280
Sequences flagged as poor quality0
Sequence length51
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG574150.3940285273496906TruSeq Adapter, Index 14 (97% over 44bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT334160.2293278284406037No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG73100.038.9383445
TTCCGTA73750.038.92555236
TCCGTAT75100.038.4055737
CTCGTAT74650.038.2467344
TATCTCG77850.036.9911241
CCGTATC78150.036.8203338
CGTATCT78700.036.5630139
GTTCCGT79700.036.47134835
AGTTCCG81700.035.22393434
AGTCACA82100.035.2201428
ACGTCTG88500.035.1324115
GTATCTC83450.034.53575540
GTCTGAA90050.034.3777817
ATCGGAA90250.034.2765542
GTCACAG85750.033.8521829
TCACAGT85300.033.81926330
CGTCTGA92600.033.45538716
TCGGAAG92800.033.3104443
CACGTCT93750.033.2128714
ACAGTTC86900.033.14206332
Produced by FastQC (version 0.11.2)