FastQCFastQC Report
Tue 29 Sep 2015
C7W8DACXX l05n01 32nm-1.3410000003515e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7W8DACXX l05n01 32nm-1.3410000003515e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16810046
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3442752.0480312784390953No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2173741.2931196024091784No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG1628050.9684982420631092TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG197000.041.7242845
CTCGTAT197650.041.59485644
ACGTCTG211250.040.6396415
AGTCACA204600.040.24302328
AATCTCG201500.039.94960841
CACAGTC207850.039.63377431
GTCACAG209950.039.39974629
CACGTCT218400.039.37098314
ACAGTCA208000.039.32111432
GTCTGAA217950.039.16324617
TCACAGT210650.039.1292530
CGTCTGA220100.038.90334716
ATCGGAA219950.038.8787352
ACACGTC220850.038.8323513
TCTCGTA213300.038.66935743
TCGGAAG224550.038.072273
CAGTCAC219500.037.93158727
AGTCAAC214400.037.6121334
AACAATC209800.037.58631538
CAGTCAA216050.037.34571533
Produced by FastQC (version 0.11.2)