FastQCFastQC Report
Tue 29 Sep 2015
C7W8DACXX l05n01 2nm-3.34100000035035.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7W8DACXX l05n01 2nm-3.34100000035035.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12324989
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC6784635.504775704059452TruSeq Adapter, Index 3 (100% over 51bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175340.14226381865330673No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTAGG736250.044.22106632
CACTTAG738250.044.17190631
TCGTATG750100.044.11950343
TTAGGCA732300.044.05709534
CTCGTAT750700.044.03678542
CTTAGGC740650.043.9917833
ACGTCTG764050.043.885715
TAGGCAT735300.043.87648835
TCACTTA743900.043.8426430
GTATGCC756050.043.74469845
CGTCTGA766400.043.71297516
CACGTCT767450.043.67368714
ATCGGAA762950.043.6511462
ACACGTC767450.043.6150613
AGTCACT753150.043.6034828
GTCACTT758450.043.58360729
GTCTGAA765550.043.56754317
TCGGAAG766700.043.449383
CGTATGC761500.043.42999344
TCTCGTA761050.043.42955441
Produced by FastQC (version 0.11.2)