FastQCFastQC Report
Tue 29 Sep 2015
C7W8DACXX l05n01 2nm-1.34100000034fa3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7W8DACXX l05n01 2nm-1.34100000034fa3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14713890
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC455070.3092791912947562TruSeq Adapter, Index 1 (100% over 51bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229260.1558119572730257No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG60500.036.55607643
CTCGTAT62400.035.54958342
ACGTCTG68850.033.6893415
GTCACAT70100.031.52340329
ACACGTC74050.031.35396213
ATCGGAA73550.030.9859312
CACGTCT75600.030.7408814
AGTCACA72000.030.62902628
CGTCTGA75900.030.5304516
TCACGAT72100.030.23695835
CGTATGC74100.029.87719244
TCTCGTA74800.029.8072341
GTCTGAA78050.029.71827117
TCGGAAG78500.029.2039993
GTATGCC76500.028.97242745
ATCACGA77450.028.03437834
CAGTCAC80000.027.93179727
TCACATC79650.027.7432930
CACACGT83900.027.5925112
GCACACG89750.026.09480511
Produced by FastQC (version 0.11.2)