FastQCFastQC Report
Tue 29 Sep 2015
C7W8DACXX l05n01 16nm-2.34100000035134.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7W8DACXX l05n01 16nm-2.34100000035134.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17828236
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC14572478.173814840683061TruSeq Adapter, Index 11 (100% over 51bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4327512.427334930948861No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2494831.3993700778921707No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACCTCTCGTATGCC329950.18507159093025244TruSeq Adapter, Index 11 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1684300.044.156182
ACGTCTG1691200.044.14098415
TCGGAAG1685150.044.1138843
AGTCACG1648150.044.06464828
CTCGTAT1636950.044.0102242
TCGTATG1639200.044.00467743
CGTCTGA1695050.043.9650716
ACACGTC1697100.043.96366513
GTCTGAA1687750.043.95395317
CACGTCT1699750.043.95335814
CGGAAGA1695650.043.7717254
GTATGCC1646100.043.732845
GCTACAT1602400.043.72496435
ACGGCTA1645100.043.71095732
TCTCGTA1649600.043.68982741
GCACACG1710600.043.64958611
CAGTCAC1666700.043.61337327
CGTATGC1651350.043.58886744
TCCAGTC1691300.043.48461525
AGCACAC1713850.043.48148310
Produced by FastQC (version 0.11.2)