FastQCFastQC Report
Tue 29 Sep 2015
C7W8DACXX l05n01 16nm-1.34100000035127.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7W8DACXX l05n01 16nm-1.34100000035127.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11350914
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC2414172.126850754045005TruSeq Adapter, Index 10 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG279200.043.4019443
CTCGTAT281100.043.11411342
ACGTCTG287950.042.83852415
GTCACTA280950.042.62008329
ATCGGAA290000.042.5742952
CTTATCT282850.042.50553537
GCTTATC282950.042.4666636
TATCTCG285100.042.33579639
CGTCTGA290300.042.313516
TAGCTTA281100.042.2404434
CACGTCT292750.042.17437414
TTATCTC286350.042.12742238
TCGGAAG294500.041.9619523
ACACGTC294000.041.956813
TCACTAG285350.041.9467530
AGTCACT287700.041.92518228
AGCTTAT283950.041.84982735
GTCTGAA292350.041.80131517
CGTATGC289850.041.79187444
GTATGCC290800.041.68204545
Produced by FastQC (version 0.11.2)