FastQCFastQC Report
Tue 29 Sep 2015
C7W8DACXX l03n01 sv88.34100000034d3b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7W8DACXX l03n01 sv88.34100000034d3b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47532853
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC9950552.0934047447141455TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1214200.043.2576181
CGCCAAT1148850.043.1716833
ATCGGAA1220650.043.1363372
CGTCTGA1210000.043.13297316
ACACGTC1212550.043.12909713
TATCTCG1181350.043.09504339
CTCGTAT1180050.043.08409542
GTCACGC1183300.043.06672729
TCTCGTA1181200.043.06323641
ACGTCTG1216950.042.9920115
TCGTATG1186450.042.96602243
ACGCCAA1148800.042.96391732
CGTATGC1185300.042.9046944
TCGGAAG1229800.042.8040163
TCACGCC1187050.042.7294530
ATCTCGT1193550.042.6479540
AGTCACG1190650.042.63774528
CGGAAGA1230500.042.6370624
CACGTCT1230600.042.5736314
CACGCCA1177100.042.50931