FastQCFastQC Report
Tue 29 Sep 2015
C7W8DACXX l03n01 sv87.34100000034d04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7W8DACXX l03n01 sv87.34100000034d04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49196391
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC5022151.0208370772563378TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA599050.042.59065241
CTCGTAT599700.042.5592842
GATCTCG598650.042.42825339
TCGTATG604350.042.3012143
CGTATGC605300.042.1865544
ATCTCGT608500.041.83682340
CGTCTGA623000.041.6597916
ACACGTC628650.041.470913
ACGTCTG630150.041.3977215
GATCGGA641950.040.82171
ATCGGAA646600.040.5845532
CACGTCT645200.040.4983214
TCGGAAG654450.040.1316383
CGGAAGA657500.039.8291444
GTATGCC643350.039.77383845
GCACACG661650.039.51472511
CACACGT667050.039.17798212
AGTGATC675950.037.1422636
GTGATCT686200.036.91306337
GTCACAC706150.036.0337329
Produced by FastQC (version 0.11.2)