FastQCFastQC Report
Tue 29 Sep 2015
C7W8DACXX l03n01 sv86.34100000034ce9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7W8DACXX l03n01 sv86.34100000034ce9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52449948
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC14414612.7482601126697017TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC1785200.043.13419313
CGTCTGA1787900.043.05428316
ACGTCTG1798500.042.89183815
CACGTCT1808750.042.6599714
TCTCGTA1748050.042.5469841
CTCGTAT1746450.042.5421642
AATCTCG1720250.042.51795639
TCGTATG1752850.042.4615443
CGTATGC1745100.042.4323344
ATCGGAA1856500.042.3955232
GCACACG1827100.042.36171311
GATCGGA1857700.042.333691
ATCTCGT1750100.042.2490340
CACACGT1828750.042.24230612
TCGGAAG1866150.042.1479233
CGGAAGA1863150.042.0539974
GTATGCC1775500.041.7148545
GACCAAT1759050.040.89948335
ACCAATC1770000.040.56138236
ACTGACC1844550.040.33122632