FastQCFastQC Report
Tue 29 Sep 2015
C7W8DACXX l02n01 handrlaz15hpfercc3.34100000034eda.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7W8DACXX l02n01 handrlaz15hpfercc3.34100000034eda.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18240601
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT279980.15349274949876926No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT91850.015.309257520
GCAGTGG116650.014.7737313
AGCAGTG122400.014.0250212
AGTGGTA127400.013.350535
GTGGTAT128000.013.3055266
AGAGTAC129600.012.86371719
CGCAGAG133950.012.58023616
GGTATCA140850.012.2353968
AGTACTT115650.012.19762221
GTACGGG47600.012.05276121
TGGTATC142150.012.0443667
AGTACGG49250.011.69464520
CAGAGTA145650.011.50788518
GCAGAGT145900.011.50358617
GTACTTT124100.011.49389522
CAGTGGT150700.011.4804594
ACGCAGA148900.011.37758315
TACGGGG31600.011.32034722
GTATCAA152250.011.3044729
GAGTACG50550.011.2603719
Produced by FastQC (version 0.11.2)