FastQCFastQC Report
Tue 29 Sep 2015
C7W8DACXX l02n01 handrlaz15hpfercc1.34100000034db1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7W8DACXX l02n01 handrlaz15hpfercc1.34100000034db1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20073236
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT296150.14753475722598985No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT92750.014.82112820
GCAGTGG116750.014.1442583
AGCAGTG127000.012.9854312
AGTGGTA127800.012.8156765
GTGGTAT129700.012.6973216
AGAGTAC130200.012.44157819
CGCAGAG132900.012.15468416
AGTACTT119050.011.69806821
GGTATCA142300.011.6520838
TGGTATC142750.011.520797
CAGTGGT149150.011.3582844
CAGAGTA146400.011.15688718
GCAGAGT149050.011.04900917
ACGCAGA149500.010.86526315
TCAACGC151050.010.84305412
GTACTTT130250.010.74393522
GTATCAA156400.010.6447629
AACGCAG158100.010.44494614
CAACGCA157900.010.386909513
ATCAACG162150.010.14241511
Produced by FastQC (version 0.11.2)