FastQCFastQC Report
Tue 29 Sep 2015
C7W8DACXX l02n01 handrlacz15hpfercc2.34100000034e2d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7W8DACXX l02n01 handrlacz15hpfercc2.34100000034e2d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16443240
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT268560.16332547600108008No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT83050.015.63119720
GCAGTGG106100.014.0797263
AGCAGTG112350.013.4569192
AGTGGTA110950.013.3628665
GTGGTAT113500.013.1617936
AGAGTAC111650.013.138473519
CGCAGAG115600.012.74753816
AGTACTT103800.012.54981421
GTACGGG36800.012.28865321
GGTATCA122350.012.2281448
GAGTACG37100.012.06799819
ACGCAGA125600.011.84008115
TGGTATC126900.011.7897037
AGTACGG38800.011.77118920
CAGAGTA126950.011.71435118
GCAGAGT127850.011.68450217
TCAACGC128550.011.60337412
CAGTGGT130600.011.4728834
GTACTTT114050.011.4218922
AACGCAG133900.011.1901314
Produced by FastQC (version 0.11.2)